Extracellular fibrinogen: the bad and the ugly in DMD

Having been for a long time in the radar of Pura Muñoz Cánoves from the CEXS-UPF, she could now demonstrate that the severity in muscular dystrophy is fibrinogen dependent. This fibrin precursor, which is never located outside of the vascular compartment in healthy muscle, is deposited in the extracellular matrix in mdx mice, the animal model for Duchenne muscular dystrophy (DMD). In their paper published in Human Molecular Genetics the researchers could furthermore show that the mechanisms supporting disease progression depend on the αMß2-binding motif of fibrinogen. Once this motif was experimentally eliminated from the fibrinogen gamma chain, this was sufficient … Continue reading Extracellular fibrinogen: the bad and the ugly in DMD

Repairing muscle after injury

Repairing a tissue after an injury requires the infiltration of inflammatory cells and the activation of the resident stem cells, which will restore the damaged tissue. But for full tissue recovery to happen, the inflammation that is first necessary must be resolved. The Myogenesis research group at the CEXS-UPF, led by Pura Muñoz-Cánoves, has recently provided evidence of how this happens. For the inflammation to disappear, macrophages (a type of immune cells that are involved in the healing of muscle and other tissues) must switch from a pro-inflammatory to an anti-inflammatory phenotype. While it is known that disturbing the interactions … Continue reading Repairing muscle after injury

Muscle or mosaic?

Muscle or mosaic? This image by Francesc Sànchez Corredera, from Esther Barreiro’s lab on Molecular mechanisms of lung cancer predisposition (IMIM-Hospital del Mar), is a 3m thick sample of a Guinea pig diaphragm muscle, dyed with an Anti-Myosin Type II antibody and amplified 20 times. The protein myosin II is expressed in fast muscle fibres, but not in slow ones. This is why in this image we can see the fast muscle fibres in which myosin II is present dyed in brown, while the slow muscle fibres are negative (not dyed and therefore white). This way, researchers can count the number of … Continue reading Muscle or mosaic?