DNA isolation method may affect methylation results

Researchers from the neurovascular research group at the IMIM have recently published a paper showing that, when studying DNA methylation, the protocol used for DNA isolation can affect the results. In the paper published in the Open Access journal PLoS One, Jaume Roquer and the rest of the authors – all part of the Spanish Stroke Genetics Consortium – used three different commercial kits for DNA extraction for each sample, and then quantified the global DNA methylation (GDM) by a luminometric methylation assay (LUMA). In the 580 samples analysed, they found significant differences in GDM in the same samples between the three … Continue reading DNA isolation method may affect methylation results

Horses with spots and giraffes with stripes

Shigeru Kondo (Institute of Frontier Biosciences, Osaka University, Japan) gave one of the last talks at the “Computational approaches to networks, cells and tissues” meeting that took place this week  at the PRBB Auditorium. Co-organised by James Sharpe (CRG) and Hernán López-Schier (HZM), the meeting was supported by QuanTissue, a collaborative European network to bridge the gap between the traditional developmental cell biology, biophysics and systems biology. And so it did! Most of the nearly 200 participants were physicysts or mathematicians, as one could tell from their presentations and posters full of complicated mathematical formulae. But the subjects they studied … Continue reading Horses with spots and giraffes with stripes