3D embryos

3D embryo

The image, by Laura Quintana (CRG) shows a mouse embryo with double antibody staining for Neurofilament (blue) and E-cadherin (green) proteins, labelling the nervous system and the internal organs respectively. It was captured using OPT (Optical Projection Tomography), a microscopy technique for 3D imaging of specimens of between 1mm-1cm.
The OPT scanner projects light through the cleared specimen (a specimen made transparent using a clearing solution), taking 400 images as it rotates 360º. Those images are reconstructed using computer software to create a 3D image of the whole specimen. Since it uses diverse UV-filters and white light, multiple labelling can be simultaneously captured, as it shows in the image.


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