3D embryos

3D embryo

The image, by Laura Quintana (CRG) shows a mouse embryo with double antibody staining for Neurofilament (blue) and E-cadherin (green) proteins, labelling the nervous system and the internal organs respectively. It was captured using OPT (Optical Projection Tomography), a microscopy technique for 3D imaging of specimens of between 1mm-1cm.
The OPT scanner projects light through the cleared specimen (a specimen made transparent using a clearing solution), taking 400 images as it rotates 360º. Those images are reconstructed using computer software to create a 3D image of the whole specimen. Since it uses diverse UV-filters and white light, multiple labelling can be simultaneously captured, as it shows in the image.

Advertisements

Tags: ,

About PRBB Communications

We lead biomedical translational research in Southern Europe

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

%d bloggers like this: